The basic principle is sterilization at over 100 ° C in saturated and submerged water vapor. In this method; Hydrolysis of the proteins of the high energy microorganisms carried by the pressurized water vapor, and the action takes place in a short time. Vehicles called autoclaves are used for this purpose. Removal of air in the autoclave is achieved either by direct air saturated steam, by displacement of the vapor or by pre-vacuuming. Once the air has been pre-vacuumed, saturated steam is fed into the medium. Mechanical removal of air with a vacuum before steam is applied will remove the suspicion of sterilization, since only vapor removal of air does not provide a satisfactory sterilization. That is, the use of pre-vacuum autoclaves increases the safety of sterilization.

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